WebThe resulting cell pellet is used for hepatocyte purification as described above. The supernatant containing non-parenchymal cells is centrifuged at 150xg for 5 minutes, and the pellet is wash twice with DMEM medium. The final cell suspension is laid on the top of four OptiPrepTM gradients (1.085, 1.058, 1.043, 1.034) and WebExperiments in Chapter 3.1 including hepatocyte purification and RNA extraction were performed with assistance from Drs. Shu-Huei Tsai and Avinash Thakur. RNA integrity analysis and RNA-seq library construction and sequencing were performed by Ryan Vander Werff at the UBC Biomedical Research Centre.
Purification of Hepatocytes and Sinusoidal Endothelial Cells from …
Web5 apr. 2024 · c-Met (hepatocyte growth factor receptor, HGFR) is a type of receptor tyrosine kinase which belongs to the MET family. c-Met exsists in the surfaces of various epithelial cells. Hepatocyte growth factor (HGF) is the ligand for c-Met. HGF belongs to the soluble cytokine family and is also a member of the plasminogen-related growth factor family. Web- Toxin purification from plant extracts - NMR analysis of purified extracts - Development and validation ... Huestis MA, Lattanzio L, Marsella LT, Tagliabracci A, Carlier J*, and Busardò PB. Human hepatocyte 4-acetoxy-N,N-diisopropyltryptamine metabolite profiling by reversed-phase liquid chromatography coupled with high-resolution tandem ... go string readline
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Web15 feb. 1991 · GOHDA, E, PURIFICATION AND PARTIAL CHARACTERIZATION OF HEPATOCYTE GROWTH-FACTOR FROM PLASMA OF A PATIENT WITH FULMINANT HEPATIC-FAILURE, JOURNAL OF CLINICAL INVESTIGATION 81: … Web5). (A) RT-PCR corresponding to each step of hepatocyte purification. mRNA was prepared from either splenocytes (lane 6) or 104 hepatocytes at each step of the purification (lanes 2 to 5), reverse transcribed and amplified using oligo-nucleotides specific for MHC class II I-Ab or for mouse -actin. (B) Hepatocytes purified from steps 1, 2 and 3 … Web2 jul. 2014 · Both HAI-1 and HAI-2 were initially purified from conditioned medium of the MKN45 human gastric carcinoma cell line as an efficient inhibitor of HGFA [ 62, 63 ]. These two HAIs are type I transmembrane proteins with two Kunitz-type serine protease domains (bikunin) in the extracellular domain. chief nettle carrier history