Web30 set 2015 · Immunohistochemical staining of 5-μm sections was performed with primary antibodies against CD68 (1:100, clone KP-1; Dako, Glostrup, Denmark) and CD31 (1:100, clone JC70A; Dako, Glostrup) for 60 min at room temperature, followed by incubation with peroxidase-labeled anti-mouse IgG for 60 min. Staining was completed with a 10-min … WebJC70A, RTU (Dako Omnis) // 12 ml GA611 // FLEX Monoclonal Mouse Anti-Human Smooth Muscle Actin, Clone 1A4, RTU (Dako Omnis) // 12 ml GA613 // FLEX Monoclonal Mouse …

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WebBackground. The treatment of middle ear cholesteatoma requires surgical treatment and the reconstruction of the temporal bone, which represents an ongoing problem. Otologists have focused on the research of materials allowing an airy middle ear and the preservation of hearing function to reconstruct the temporal bone. Methods. This study evaluated the … WebThereafter, the samples were rinsed twice with deionized water, followed by incubation with the respective primary antibody: monoclonal mouse anti-human CD31 for endothelial cell staining (clone JC70A, Dako, Santa Clara, CA, USA, 1:50), monoclonal mouse anti-collagen IV as a marker for basement membrane (clone COL-94, abcam, Cambridge, UK, 1:250), … companies that have a monopoly on the market

Dako antibody summary

Web22 feb 2013 · Each section was blocked with DAKO Protein block (X0909) for 10 min at room temp and incubated with primary antibody for 1 hour at room temp (GFAP 1∶50, Vimentin 1∶100, IDH1 R132H 1∶20). The Universal R.T.U. Vectastain Elite ABC Kit (Vector Laboratories, Burlingame, CA, USA) was used for CD105 (4G11) (Leica Microsystems, … Webanti-human antibody (Dako Corporation, Carpinteria, incubated for 30 minutes. For CD31 immunohisto-chemistry, monoclonal mouse anti-human antibody clone JC70A (Dako Corporation, Carpinteria, CA) was used at a dilution of 1:25 and was incubated for 60 minutes. Detection of primary antibody binding was performed WebMonoclonal Mouse Anti-Human CD31, Endothelial Cell, Clone JC70A, Concentrated Antibody for Manual Use, Unconjugated, Culture supernatant, Immunohistochemistry, 1 mL. eaton qcd breaker