Optithaw rodent hepatocyte isolation kit
WebDec 23, 2016 · Class 3 Device Recall Optilite IgG4 Kit. A change to the lower end of the measuring range and product insert did not reflect the change.The measuring range … WebThis is a serum-free medium for the long-term maintenance of hepatocyte phenotypic expression including the active and inducible forms of Cytochrome P450 and active phase II enzymes. This medium should be supplemented with 2 mM L-glutamine and 5 mL (1X) penicillin-streptomycin prior to use. Features The Hepatocyte Product Line
Optithaw rodent hepatocyte isolation kit
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WebIt has been optimized for a high yield of non-parenchymal mouse liver cells, including liver sinusoidal endothelial cells (LSECs) and Kupffer cells, while preserving most cell surface epitopes. Dissociated cells can be cultured or isolated using MACS ® Technology. Product name Size Quantity List price 130-105-807 For research use only WebApoptosis kits enable analysis of active caspases in cells using a cell permeable and non-cytotoxic reagent. We offer kits for detecting apoptotic cells by flow cytometry.
WebSprague–Dawley Rat Primary Hepatocytes - Plateable . Catalog No. RA-6224F. Suggested Medium: Hepatocyte Medium /w Kit (125 ml) Catalog No. M1365 . Product Description. Rat Primary Hepatocytes are derived from the liver of laboratory Sprague–Dawley rat. Cells are freshly isolated and frozen at passage 0. Each vial contains 3 x10 6 cells ... WebOptiThaw Hepatocyte Kit
http://comparativegenomics.illinois.edu/sites/default/files/publications/Poster_Porcine%20drug%20metabolism%20and%20toxicity%20in%20vitro%20model%20utilizing%20pHCC_2016.pdf WebThaw cryopreserved hepatocytes in 37°C water bath for <2 min. Wipe the vial with 70% alcohol in hood; pour or use wide-bore pipette tip to transfer hepatocytes into CHRM® Medium (for human) or Williams Medium E and Hepatocyte Plating Supplement Pack (for animal). Centrifuge at room temperature: Human hepatocytes, 100 x g for 10 min.
WebIsolation of primary murine hepatocytes Preparation: 1. Animal, 6-8wks male C57BL/6 mice 2. Instruments 1) BIO RAD peristaltic pump, model# EP-1 Econo Pump 2) water bath (37°C) 3) 24GA 0.75IN 0.7x19mm yellow, closed IV catheter (BD 383511) 4) Surgical forceps, scissors 5) 100/70µm cell strainer (BD) Medium: 1) Perfusion medium (GIBCO # 17701-038)
WebMay 28, 2024 · Isolation, cryopreservation and thawing of hepatocytes Isolation, cryopreservation and thawing of hepato- ... al., 1999b) using a commercially available kit (DCS Innovative Diagnostik Systeme, Hamburg, Germany). Briefly, 150 L of cell suspension containing 20, ... in rat hepatocytes, discrepancies between individual animals were … list of wonder woman characters wikipediaWebMar 19, 2015 · Techniques of hepatocytes isolation started in rat liver and subsequently applied to human and several other species. The technique was refined by Berry and Friend and ... BrdU assay was performed as per manufacturer’s protocol using BrdU cell proliferation assay kit (Cat No. Q11A58, Calbiochem, USA). Hepatocytes were seeded on … im not a good person pat the bunnyWebThe Worthington Hepatocyte Isolation System has been developed to provide researchers with a reliable, convenient, and consistent hepatocyte cell isolation system. By using the pre-optimized combination of enzymes contained in this kit, it is possible to minimize the lot-to-lot variation and improve the quality of the isolated hepatocytes. list of wood densitiesWebIsolation from a normal male rat (BW=350-550g) yields 2-3x108 hepatocytes with the purity and viability exceeding 92%, and 1.8-2.5x108 HM with the purity > 83% and the viability >95%. HM are further purified by the adherence method and cultured in PRMI containing 5% FCS and antibiotics at the density of 3-4x106 cells per 100 mm dish. After ... list of women\u0027s hairstylesWebApr 5, 2024 · The isolated primary rat hepatocytes consistently exhibit high cell viability (85%-95%), yield (2-5 x 10 8 cells per 200-300 g rat) and functionality (albumin, urea and CYP activity). The procedure was complemented by an integrated perfusion system, which is compact enough to be set up in the laminar flow hood to ensure aseptic operation. im not a good person pat the bunny lyricsWebOptiThaw CryostaX Rodent Hepatocyte Kit. Specifications; Datasheet; Product name OptiThaw CryostaX Rodent Hepatocyte Kit Catalog number K8800-KIT (1 kit) Description … im not a human lyricsWebMar 26, 2013 · RNA isolation Total RNA was isolated from cultured mouse hepatocytes using TRIzol reagent with the RNeasy kit according to the manufacturer's protocol. Total cellular levels were measured on a spectrophotometer, and the quality of each RNA preparation was determined with a bioanalyzer. Extracted RNA was stored at -80℃ [3]. i m not a good person