Phospho-mtor antibody
WebULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to … Webadult animals (c) Immunoprecipitation (IP) with mTOR antibody efficiently extracts mTOR complexes from prepared lysates. mTOR is highly enriched in the IP fraction but is absent …
Phospho-mtor antibody
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WebThe Phospho-mTOR (Ser2481) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to MTOR. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-mTOR (Ser2481) Antibody has been validated for the following applications: Western Blot. WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. Alias: Fold/Unfold
WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. … WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal ...
WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. We previously demonstrated that the mammalian target of rapamycin (mTOR) inhibitor rapamycin suppressed orofacial formalin injection-induced nociception; however, the … WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for …
WebmTOR is a member of the PI3 family of kinases and plays a critical role in regulating cell proliferation and metabolism in response to nutrient availability, cellular energy status, …
WebImmunofluorescent analysis of Phospho-mTOR (Ser2481) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X … flip or flop season 10 episode 5WebAnti-phospho-mTOR (Thr2446) Antibody Upstate®, from rabbit Synonym (s): FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1 eCl@ss: 32160702 NACRES: NA.41 Pricing and availability is not currently available. Properties biological source rabbit Quality Level 100 antibody form affinity purified immunoglobulin flip or flop season 2 watch onlineWebThis Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse phospho-AKT/ GSK3 beta / mTor and total-AKT/ GSK3 beta / mTor. For each target, a capture antibody has been coated onto microwells. greatest hits cornwallWebRabbit recombinant monoclonal mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free. Validated in WB, IHC, Dot and tested in Mouse, Human. flip or flop season 7 episode 4WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. greatest hits contactWebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1811 publications. Validated for WB, IP, IF. Available in 2 sizes. Highly … greatest hits countdownWebKey features and details Rabbit polyclonal to mTOR (phospho S2448) Suitable for: WB, IHC-P, ICC/IF Reacts with: Human Isotype: IgG You may also be interested in Primary Anti … flip or flop season 3